pcr laboratory setup
PCR laboratory
The PCR laboratory is mainly located in the laboratory and pathology department. It can be amplified and analyzed in vitro. To diagnose the prevalence of infectious diseases, cancer, AIDS, hepatitis A, hepatitis B, tuberculosis, syphilis, rubella and other patients. It is usually used for early diagnosis of diseases and various treatment stages. Through the DNA gene tracking system, can quickly grasp the virus content in the patient's body, and its accuracy is as high as nanometers.
1.Reagent preparation area
Function: It is mainly used for the preparation of storage reagents, the packaging of reagents and the preparation of the main reaction mixture. The reagents and materials used for specimen preparation should be transported directly to this area, and should not pass through other areas. Reagent raw materials need to be stored in this area, and the required storage reagents are prepared in this area.
Reagent preparation room: configuration of experimental reagents.
Reagent configuration steps: Weigh solid medicines with a balance or pipette to take liquid reagents (mix), add ultrapure water to dissolve, can configure electrophoretic solution, sodium acetophenate solution, reagent configuration, can be operated in an ultra-clean workbench.
| Category | Selection Purpose |
| Balance | Used for accurate weighing of various reagents |
| Ultra-clean workbench | All operations involving bacteria must be done under the ultra-clean workbench. |
| Pipette | Accurately measure a specific volume of solution 20-100ul |
| Water purifier | Ultra pure water 18.2ΩM.CM |
| Medicine refrigerator | Short-term storage |
| Reagent low temperature refrigerator | Optional 2-8o,-20℃ |
| Mixer | Need to mix before transferring the sample |
| Disinfection vehicle | Space environment disinfection |
| Sterilization pot | Sterilization and sterilization of laboratory items, reagents, culture media |
| PH meter | Configuration reagent is accurate measurement of PH value |
2. Sample preparation area
Function: preservation of clinical specimens, nucleic acid (RNA, DNA) extraction, storage and DNA synthesis when added to amplification reaction tubes and RNA determination.
Sample preparation room: nucleic acid extraction and purification
Extraction: Pipette the sample after mixing, add 0.5% SDS and protease to digest at 70°C (constant temperature water tank), add phenol and chloroform
| Category | Selection Purpose |
| Biological safety cabinet | To prevent the spread of harmful suspended particles and aerosols |
| Sample Refrigerator | Store samples and DNA in -20°C, -80°C |
| High-speed tabletop refrigerated centrifuge | It is used to collect microbial cells, cell debris and other sediments. Some samples require low temperature environment because they are not stable at normal temperature. |
| Water bath or metal bath | Used for temperature control of water bath during DNA hybridization |
| Pipette | Accurately measure a specific volume of solution |
| Tips | Matching pipettes |
| Ultraviolet lamp or disinfection vehicle | Space environment disinfection |
| Mixer | Need to mix before transferring the sample |
| Ultra-clean workbench | All operations involving bacteria must be done under the ultra-clean workbench. |
| Low temperature press | For shaking culture of E. coli and yeast |
| Magnetic stirrer | Accelerates the dissolution of solid content |
3. Nucleic acid amplification area
Function: Mainly DNA amplification, prepared DNA template and synthesized DNA and main reaction mixture, prepared into reaction mixture, etc., can also be carried out in this area. In PCR measurement, the reaction tube is usually opened after amplification.
| Category | Selection purpose |
| Gene Amplifier | Genetic testing DNA/RNA amplification |
| Fluorescence quantitative PCR instrument | Nucleic acid quantitative analysis |
| Nucleic acid extractor | Sample DNA/RNA extraction |
| Pipette | Accurately measure a specific volume of solution |
| UV lamp or disinfection vehicle | Space environment disinfection |
| Ultra-clean workbench | Operations involving bacteria must be completed under an ultra-clean workbench. |
| Water purifier S | For PCR, DNA sequencing requires ultrapure water |
4. Product analysis area
Function: preservation of clinical specimens, nucleic acid (RNA, DNA) extraction, storage and DNA synthesis when added to amplification reaction tubes and RNA determination.
Sample preparation room: nucleic acid extraction and purification
Extraction: Pipette and mix the sample, add 0.5% SDS and protease to digest at 70°C (constant temperature water tank), add benzene and chloroform
| Category | Selection purpose |
| Electrophoresis | Horizontal electrophoresis is used for nucleic acid DNA/RNA detection, vertical electrophoresis is used for protein detection, and transfer electrophoresis is used to transfer protein to the membrane for western detection. |
| Gel imaging system/UV detector | For nucleic acid agar electrophoresis and protein polyacrylamide observation and photographing; UV analyzer is used for nucleic acid agar electrophoresis gel observation after staining, can not be connected to a computer |
| Electric stove | Electrophoresis agar gel melted by heating |
